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in studies of circulating cell free DNA
The Spike II assay which is used for detecting the Universal DNA Spike II template
Features High sensitivity - optimized for two-step quantitative RT-PCR Broad dynamic range Unbiased cDNA synthesis - complete 5’ to 3’ RNA sequence representation Easy reaction assembly
analyzing a sample with TATAA ALU-60 and TATAA ALU-187 assays
TATAA Universal DNA Spike 500 bp Reactions/SYBR or Probe:TATAA Universal DNA Spike 500 bp 1000 rxn probe FAM respectivelyControl for qPCR inhibition in samples with free circulating DNA or in degraded material The Universal DNA Spike from TATAA Biocenter is easy to use and an very effective tool for quality control of the qPCR experimental workflow. DNA Spike is used in the qPCR reaction to detect any inhibition. In addition TATAA Universal RNA Spike I and II can be used to detect any inhibition during the cDNA synthesis reaction. In cases where predominantly short
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