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The assay amplifies a 286-base region towards the 3’ end of the synthetic template
generating amplicons of 60 and 187 bp
In addition TATAA Universal RNA Spike I and II can be used to detect any inhibition during the cDNA synthesis reaction
primers and probes
and diagnostics
TATAA Alu Assays Amplicon Length (bp):ALU-187 The assay amplifies a 286-baseAlu Assays Alu elements are short stretches of about 300 bp that are unique to the human genomic DNA (gDNA). There are over one million Alu elements interspersed throughout the genome constituting about 10. 7% of the bases. The TATAA Alu Assays target consensus Alu sequences making them exceptionally sensitive for human gDNA. In fact, the assays are so sensitive that we rarely find a negative sample! Ultrasensitive detection and semi quantification of
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